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Prevalence of Viruses in Commercial Wine Grape Vineyards in Georgia

P. M. Brannen, C. Michael Deom, O. J. Alabi, and R. A. Naidu

December 2018

Survey

Virus diseases are major constraints to Vitis vinifera wine grape production worldwide. Grapevine leafroll-associated virus 3 (GLRaV-3) was first confirmed in Georgia in 2008. The negative impacts of GLRaV-3, such as decreased fruit yield and quality, were confirmed from samples taken in 2009 and 2010. In light of these findings, studies were initiated to determine the prevalence and types of grapevine viruses present in vineyards in Georgia. Five vineyard blocks were visited during August and September of 2011 and 2012. Leaf samples were collected from a total of 50 grapevines showing symptoms of and suspected as being infected by grapevine leafroll disease (GLD). Samples from individual grapevines were extracted and tested separately for 20 grapevine viruses listed in standard virus indexing programs. Reverse transcription polymerase chain reaction (RT-PCR) or PCR, depending on the nature of the virus genome, was used to detect these viruses with species-specific primers. The results showed the presence of GLRaV-1, -2, -3, and -4 (strains 4, 5, and 6), grapevine virus B, grapevine rupestris stem pitting-associated virus, and grapevine red blotch virus. Many vineyards had multiple viruses detected from individual grapevines showing typical GLD or GLD-like symptoms.

doi:10.1094/PHP-07-18-0040-S

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